Not really. You could in theory emulate the ROM chips, but that would still involve hardware needing to be attached to the Amiga's mainboard via the ROM or CPU sockets. The closest you might get is one of the flash ROM devices now available (or currently in the works) - they still need hardware to be installed in the ROM sockets but at least then they can also be used for loading alternative Kickstarts as well (and possibly switching between them and DiagROM).
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