Indy definitely doesn't count for me, as he's from films rather than games. Guybrush is debatable as his games were all released for the PC first - though I suspect globally the Amiga versions probably outsold the PC ones in the first year or two.
Fair point about the Settlers sprites being quite iconic - little touches like the 'yup' when you give them an instruction, the door-knocking to start the fights, and the animations of them at work, really help. I'm sure Blue Byte considered why Lemmings was so successful when developing it.
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